I think he means from primary samples of patients assumed to be infected. There is a need to have a physical virus (that meets the definition of a virus) at some stage to be able to verify and deem any genetic material found as being viral. After CPE is observed in Vero or other cells, the culture fluid is harvested. This fluid is Not purified, it contains cell debris, exosomes, fragments, enzymes, and possibly viral particles referred to as “viral isolate,” but this is a misnomer, it’s not a clean, separated viral entity. The fluid is then, treated with RNA extraction reagents
Sequenced using next-gen methods (Illumina, etc.)
The resulting short RNA fragments are computationally stitched together to create a consensus genome (in silico)
The genome is not directly sequenced from an intact virus, nor is the virus seen, isolated, or confirmed in whole form at this stage.
I think he means from primary samples of patients assumed to be infected. There is a need to have a physical virus (that meets the definition of a virus) at some stage to be able to verify and deem any genetic material found as being viral. After CPE is observed in Vero or other cells, the culture fluid is harvested. This fluid is Not purified, it contains cell debris, exosomes, fragments, enzymes, and possibly viral particles referred to as “viral isolate,” but this is a misnomer, it’s not a clean, separated viral entity. The fluid is then, treated with RNA extraction reagents Sequenced using next-gen methods (Illumina, etc.) The resulting short RNA fragments are computationally stitched together to create a consensus genome (in silico) The genome is not directly sequenced from an intact virus, nor is the virus seen, isolated, or confirmed in whole form at this stage.