In conclusion, the development of this novel in vitro model enabling real-time electrophysiological recordings within interconnected organoids represents a significant advancement in the field. This platform overcomes the limitations of assembloids by providing a means to study and characterize the functional properties of neural connections between two distinct organoids without imaging modalities. It offers new avenues for streamlined disease modeling and drug discovery, allowing the recreation of specific pathologies and the evaluation of therapeutic interventions in a more physiologically relevant context.