During a research career lasting more than 50 years, I have concluded that the following
procedures are unsuitable for studying the biology of living cells in Intact animals and plants:
subcellular fractionation; histology; histochemistry; electron microscopy; binding studies; use of
ligands; immunocytochemistry; tissue slices; disruptive techniques; dehydration; deep freezing;
freeze drying; boiling; use of extracellular markers; receptor studies; patch clamp measurements;
inadequate calibrations. The main objections to these procedures are: (i) they change the properties
of the tissues being studied grossly and significantly; (ii) they ignore the second law of
thermodynamics;(iii) they produce artefacts, many of which are two-dimensional; (iv) adequate
control procedures have never been published for them.
(also 4 attn of u/zeppelincheetah)
Dr. Harold Hillman:
https://www.big-lies.org/harold-hillman-biology/index.html
https://www.big-lies.org/harold-hillman-biology/what-price-intellectual-honesty.htm
https://www.dropbox.com/sh/297b780rk5kvbyx/AABo8GCfCdsD2E0nMHd0H623a?dl=0&preview=Harold+Hillman+2011+Cell+biology+is+in+dire+straits.pdf
https://www.dropbox.com/sh/297b780rk5kvbyx/AABo8GCfCdsD2E0nMHd0H623a?dl=0