Again that bullshit? You choose wrong forum.
SARS-CoV-2 has been isolated here, here, here, and here.
In no way this is "isolation". Isolation means to separate object of interest from any other substances.
If you want a description of virus isolation you could easily read it here Here it goes:
ChAdOx1-containing medium was clarified by centrifugation at 4000 rpm for 10 min in a benchtop centrifuge. Supernatant was then loaded into 38-ml ultraclear tubes compatible with the SW28 rotor (Beckman Coulter) and centrifuged at 100,000g for 1 hour in a Beckman Coulter Optima XPN-100 ultracentrifuge. Supernatant was discarded, and the pellet, which was slightly yellow and sticky, was resuspended in 5 ml of phosphate-buffered saline (PBS) (pH 7.4, Gibco). This 5 ml of PBS containing the ChAdOx1 from the supernatant was used to resuspend the infected HEK-293 T-REx pellet. This solution was then mixed in a 1:1 ratio with tetrachloroethylene (TCE; Sigma-Aldrich) and shaken violently to ensure thorough mixing. The virus, PBS, and TCE mixture was then centrifuged at 2000 rpm in a benchtop centrifuge for 20 min. The top aqueous layer of the solution was removed by pipetting and placed into a new tube. A second TCE extraction was then performed by adding a further 5 ml of TCE to the aqueous layer, shaking, and centrifuging, as before. This is performed to ensure maximum removal cell debris. Previous purifications that excluded this second extraction showed fatty deposits when analyzed by negative stain transmission electron microscopy and resulted in viral aggregation. Next, the top aqueous layer was extracted again, and the remainder of the purification was performed using the two-step CsCl gradient method, as previous described (42), except for the following modification: During the final extraction of the virus containing band, which should have a crisp white appearance, the band was not removed using a needle through the side of the tube but by pipetting. A P1000 pipette was used to slowly withdraw fluid from the tube, taking from the meniscus, careful not to disrupt the band. As much fluid was removed above the band as possible, then a new pipette tip was used to withdraw the band from the meniscus in as little volume as possible.
See the difference? They finally got a perfectly isolated ChAdOx1 adenovirus supposedly used in AstraZeneca vaccine for further study, including EM and other things. You could also see a EM of isolated virus with identical particles without any garbage in Supplementary Materials Fig.S1
That is isolation. Virus isolation is routine well-known procedure, that used in that paper just as a obvious intermediate step in reserach. Real scientists do it every time when they want to really study some virus. Routinely and without any problems. And that simple and obvious procedure was never done for SARS-CoV-2.
What you falsely throw here as examples of isolation of SARS-CoV-2 is not even near isolation at all. It is just a description of getting some dirty soup of parts of dead monkey cells, antibiotics, antifungicides, and God know what else, where that frauds declare presence of SARS-CoV-2 using PCR tests that can't be used to detect viruses by definition.
All EM's that provided by so-called "scientists" who declare SARS-CoV-2 "isolation" is an image of unidentifiable garbage with inclusion of few particles that posed as a virus. But they are not. Some kidney specialists do that whole fraud procedure of SARS-CoV-2 "isolation" on clean monkey cell, just for lulz, because what they saw on so-called EMs of SARS-CoV-2 they saw constantly studing kidney cells long before coronahoax. They made EM images, specially for dumb idiots, who pose usual kidney cells remnants as SARS-CoV-2. On images you could easily found exactly the same particles that was posed as SARS-CoV-2 virus. Hopefully, this scientific journal is out of propaganda control due to be very specific on kidneys, so, here you still could read that paper and fuck out with your lies.
Again that bullshit? You choose wrong forum.
SARS-CoV-2 has been isolated here, here, here, and here.
In no way this is "isolation". Isolation means to separate object of interest from any other substances.
If you want a description of virus isolation you could easily read it here Here it goes:
ChAdOx1-containing medium was clarified by centrifugation at 4000 rpm for 10 min in a benchtop centrifuge. Supernatant was then loaded into 38-ml ultraclear tubes compatible with the SW28 rotor (Beckman Coulter) and centrifuged at 100,000g for 1 hour in a Beckman Coulter Optima XPN-100 ultracentrifuge. Supernatant was discarded, and the pellet, which was slightly yellow and sticky, was resuspended in 5 ml of phosphate-buffered saline (PBS) (pH 7.4, Gibco). This 5 ml of PBS containing the ChAdOx1 from the supernatant was used to resuspend the infected HEK-293 T-REx pellet. This solution was then mixed in a 1:1 ratio with tetrachloroethylene (TCE; Sigma-Aldrich) and shaken violently to ensure thorough mixing. The virus, PBS, and TCE mixture was then centrifuged at 2000 rpm in a benchtop centrifuge for 20 min. The top aqueous layer of the solution was removed by pipetting and placed into a new tube. A second TCE extraction was then performed by adding a further 5 ml of TCE to the aqueous layer, shaking, and centrifuging, as before. This is performed to ensure maximum removal cell debris. Previous purifications that excluded this second extraction showed fatty deposits when analyzed by negative stain transmission electron microscopy and resulted in viral aggregation. Next, the top aqueous layer was extracted again, and the remainder of the purification was performed using the two-step CsCl gradient method, as previous described (42), except for the following modification: During the final extraction of the virus containing band, which should have a crisp white appearance, the band was not removed using a needle through the side of the tube but by pipetting. A P1000 pipette was used to slowly withdraw fluid from the tube, taking from the meniscus, careful not to disrupt the band. As much fluid was removed above the band as possible, then a new pipette tip was used to withdraw the band from the meniscus in as little volume as possible.
See the difference? They finally got a perfectly isolated ChAdOx1 adenovirus for further study, including EM and other things. You could also see a EM of isolated virus with identical particles without any garbage in Supplementary Materials Fig.S1
That is isolation. Virus isolation is routine well-known procedure, that used in that paper just as a obvious intermediate step in reserach. Real scientists do it every time when they want to really study some virus. Routinely and without any problems. And that simple and obvious procedure was never done for SARS-CoV-2.
What you falsely throw here as examples of isolation of SARS-CoV-2 is not even near isolation at all. It is just a description of getting some dirty soup of parts of dead monkey cells, antibiotics, antifungicides, and God know what else, where that frauds declare presence of SARS-CoV-2 using PCR tests that can't be used to detect viruses by definition.
All EM's that provided by so-called "scientists" who declare SARS-CoV-2 "isolation" is an image of unidentifiable garbage with inclusion of few particles that posed as a virus. But they are not. Some kidney specialists do that whole fraud procedure of SARS-CoV-2 "isolation" on clean monkey cell, just for lulz, because what they saw on so-called EMs of SARS-CoV-2 they saw constantly studing kidney cells long before coronahoax. They made EM images, specially for dumb idiots, who pose usual kidney cells remnants as SARS-CoV-2. On images you could easily found exactly the same particles that was posed as SARS-CoV-2 virus. Hopefully, this scientific journal is out of propaganda control due to be very specific on kidneys, so, here you still could read that paper and fuck out with your lies.
Again that bullshit? You choose wrong forum.
SARS-CoV-2 has been isolated here, here, here, and here.
In no way this is "isolation". Isolation means to separate object of interest from any other substances.
If you want a description of virus isolation you could easily read it here Here it goes:
ChAdOx1-containing medium was clarified by centrifugation at 4000 rpm for 10 min in a benchtop centrifuge. Supernatant was then loaded into 38-ml ultraclear tubes compatible with the SW28 rotor (Beckman Coulter) and centrifuged at 100,000g for 1 hour in a Beckman Coulter Optima XPN-100 ultracentrifuge. Supernatant was discarded, and the pellet, which was slightly yellow and sticky, was resuspended in 5 ml of phosphate-buffered saline (PBS) (pH 7.4, Gibco). This 5 ml of PBS containing the ChAdOx1 from the supernatant was used to resuspend the infected HEK-293 T-REx pellet. This solution was then mixed in a 1:1 ratio with tetrachloroethylene (TCE; Sigma-Aldrich) and shaken violently to ensure thorough mixing. The virus, PBS, and TCE mixture was then centrifuged at 2000 rpm in a benchtop centrifuge for 20 min. The top aqueous layer of the solution was removed by pipetting and placed into a new tube. A second TCE extraction was then performed by adding a further 5 ml of TCE to the aqueous layer, shaking, and centrifuging, as before. This is performed to ensure maximum removal cell debris. Previous purifications that excluded this second extraction showed fatty deposits when analyzed by negative stain transmission electron microscopy and resulted in viral aggregation. Next, the top aqueous layer was extracted again, and the remainder of the purification was performed using the two-step CsCl gradient method, as previous described (42), except for the following modification: During the final extraction of the virus containing band, which should have a crisp white appearance, the band was not removed using a needle through the side of the tube but by pipetting. A P1000 pipette was used to slowly withdraw fluid from the tube, taking from the meniscus, careful not to disrupt the band. As much fluid was removed above the band as possible, then a new pipette tip was used to withdraw the band from the meniscus in as little volume as possible.
See the difference? They finally got a perfectly isolated adenovirus for further study, EM and other things. You could also see a EM of isolated virus with identical particles without any garbage in Supplementary Materials Fig.S1
That is isolation. Virus isolation is routine well-known procedure, that used in that paper just as a obvious intermediate step in reserach. Real scientists do it every time when they want to really study some virus. Routinely and without any problems. And that simple and obvious procedure was never done for SARS-CoV-2.
What you falsely throw here as examples of isolation of SARS-CoV-2 is not even near isolation at all. It is just a description of getting some dirty soup of parts of dead monkey cells, antibiotics, antifungicides, and God know what else, where that frauds declare presence of SARS-CoV-2 using PCR tests that can't be used to detect viruses by definition.
All EM's that provided by so-called "scientists" who declare SARS-CoV-2 "isolation" is an image of unidentifiable garbage with inclusion of few particles that posed as a virus. But they are not. Some kidney specialists do that whole fraud procedure of SARS-CoV-2 "isolation" on clean monkey cell, just for lulz, because what they saw on so-called EMs of SARS-CoV-2 they saw constantly studing kidney cells long before coronahoax. They made EM images, specially for dumb idiots, who pose usual kidney cells remnants as SARS-CoV-2. On images you could easily found exactly the same particles that was posed as SARS-CoV-2 virus. Hopefully, this scientific journal is out of propaganda control due to be very specific on kidneys, so, here you still could read that paper and fuck out with your lies.
Again that bullshit? You choose wrong forum.
SARS-CoV-2 has been isolated here, here, here, and here.
In no way this is "isolation". Isolation means to separate object of interest from any other substances.
If you want a description of virus isolation you could easily read it here Here it goes:
ChAdOx1-containing medium was clarified by centrifugation at 4000 rpm for 10 min in a benchtop centrifuge. Supernatant was then loaded into 38-ml ultraclear tubes compatible with the SW28 rotor (Beckman Coulter) and centrifuged at 100,000g for 1 hour in a Beckman Coulter Optima XPN-100 ultracentrifuge. Supernatant was discarded, and the pellet, which was slightly yellow and sticky, was resuspended in 5 ml of phosphate-buffered saline (PBS) (pH 7.4, Gibco). This 5 ml of PBS containing the ChAdOx1 from the supernatant was used to resuspend the infected HEK-293 T-REx pellet. This solution was then mixed in a 1:1 ratio with tetrachloroethylene (TCE; Sigma-Aldrich) and shaken violently to ensure thorough mixing. The virus, PBS, and TCE mixture was then centrifuged at 2000 rpm in a benchtop centrifuge for 20 min. The top aqueous layer of the solution was removed by pipetting and placed into a new tube. A second TCE extraction was then performed by adding a further 5 ml of TCE to the aqueous layer, shaking, and centrifuging, as before. This is performed to ensure maximum removal cell debris. Previous purifications that excluded this second extraction showed fatty deposits when analyzed by negative stain transmission electron microscopy and resulted in viral aggregation. Next, the top aqueous layer was extracted again, and the remainder of the purification was performed using the two-step CsCl gradient method, as previous described (42), except for the following modification: During the final extraction of the virus containing band, which should have a crisp white appearance, the band was not removed using a needle through the side of the tube but by pipetting. A P1000 pipette was used to slowly withdraw fluid from the tube, taking from the meniscus, careful not to disrupt the band. As much fluid was removed above the band as possible, then a new pipette tip was used to withdraw the band from the meniscus in as little volume as possible.
See the difference? They finally got a perfectly isolated virus for further study, EM and other things. You could also see a EM of isolated virus with identical particles without any garbage in Supplementary Materials Fig.S1
That is isolation.
What you falsely throw here as examples of isolation of SARS-CoV-2 is not even near isolation at all. It is just a description of getting some dirty soup of parts of dead monkey cells, antibiotics, antifungicides, and God know what else, where that frauds declare presence of SARS-CoV-2 using PCR tests that can't be used to detect viruses by definition.
All EM's that provided by so-called "scientists" who declare SARS-CoV-2 isolation is an image of unidentifiable garbage with inclusion of few particles that posed as a virus. But they are not. Some kidney specialists do that whole fraud procedure of SARS-CoV-2 "isolation" on clean monkey cell. And make EM images. Where they found exactly the same particles that was posed as SARS-CoV-2 virus. Hopefully, this scientific journal is out of propaganda control due to kindey specific, so, here you still could read that paper and fuck out with your lies.
SARS-CoV-2 has been isolated here, here, here, and here.
In no way this is "isolation". Isolation means to separate object of interest from any other substances.
If you want a description of virus isolation you could easily read it here Here it goes:
ChAdOx1-containing medium was clarified by centrifugation at 4000 rpm for 10 min in a benchtop centrifuge. Supernatant was then loaded into 38-ml ultraclear tubes compatible with the SW28 rotor (Beckman Coulter) and centrifuged at 100,000g for 1 hour in a Beckman Coulter Optima XPN-100 ultracentrifuge. Supernatant was discarded, and the pellet, which was slightly yellow and sticky, was resuspended in 5 ml of phosphate-buffered saline (PBS) (pH 7.4, Gibco). This 5 ml of PBS containing the ChAdOx1 from the supernatant was used to resuspend the infected HEK-293 T-REx pellet. This solution was then mixed in a 1:1 ratio with tetrachloroethylene (TCE; Sigma-Aldrich) and shaken violently to ensure thorough mixing. The virus, PBS, and TCE mixture was then centrifuged at 2000 rpm in a benchtop centrifuge for 20 min. The top aqueous layer of the solution was removed by pipetting and placed into a new tube. A second TCE extraction was then performed by adding a further 5 ml of TCE to the aqueous layer, shaking, and centrifuging, as before. This is performed to ensure maximum removal cell debris. Previous purifications that excluded this second extraction showed fatty deposits when analyzed by negative stain transmission electron microscopy and resulted in viral aggregation. Next, the top aqueous layer was extracted again, and the remainder of the purification was performed using the two-step CsCl gradient method, as previous described (42), except for the following modification: During the final extraction of the virus containing band, which should have a crisp white appearance, the band was not removed using a needle through the side of the tube but by pipetting. A P1000 pipette was used to slowly withdraw fluid from the tube, taking from the meniscus, careful not to disrupt the band. As much fluid was removed above the band as possible, then a new pipette tip was used to withdraw the band from the meniscus in as little volume as possible.
See the difference? They finally got a perfectly isolated virus for further study, EM and other things. You could also see a EM of isolated virus with identical particles without any garbage in Supplementary Materials Fig.S1
That is isolation.
What you falsely throw here as examples of isolation of SARS-CoV-2 is not even near isolation at all. It is just a description of getting some dirty soup of parts of dead monkey cells, antibiotics, antifungicides, and God know what else, where that frauds declare presence of SARS-CoV-2 using PCR tests that can't be used to detect viruses by definition.
All EM's that provided by so-called "scientists" who declare SARS-CoV-2 isolation is an image of unidentifiable garbage with inclusion of few particles that posed as a virus. But they are not. Some kidney specialists do that whole fraud procedure of SARS-CoV-2 "isolation" on clean monkey cell. And make EM images. Where they found exactly the same particles that was posed as SARS-CoV-2 virus. Hopefully, this scientific journal is out of propaganda control due to kindey specific, so, here you still could read that paper and fuck out with your lies.